Example sentences for: anti-mouse

How can you use “anti-mouse” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membrane was incubated overnight at 4°C on a shaker, washed 4 × 15 minutes with PBS at room temperature (RT), incubated with a horseradish peroxidase-coupled anti-mouse antibody (1:5000, Dianova, Hamburg) for 3 h at and then washed again 4 × 15 minutes with PBS at RT.

  • Specimens were then transferred to room temperature, extensively washed in freshly prepared PBS, pH 7.22, and incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC Praha, Prague, Czech Republic) diluted 1:20, and preabsorbed with rat kidney homogenate to remove nonspecific background [ 46 ] . Control slides were similarly processed, but primary antibody was replaced with PBS.

  • After three washes with PBS, the cells were incubated with secondary antibody mixture of anti-mouse Ig (1:200 dilution; Huamei BG Co Ltd, Shanghai, China) for 2 h at room temperature.

  • Titrated concentrations of IgG 1 and IgG 2a myeloma proteins were used to generate a standard curve, and the IgGs were detected with the same labeled rabbit anti-mouse IgG 1 or IgG 2a antiserum.

  • The BZLF1 antibody is unlabelled and required a secondary goat anti-mouse antibody labelled with FITC.


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