Words similar to anti-mouse
Example sentences for: anti-mouse
How can you use “anti-mouse” in a sentence? Here are some example sentences to help you improve your vocabulary:
Subsequently, the sections were rinsed 4 times with .1 M PBS + .2% Triton-X and incubated for 1 hour at room temperature with the secondary antibody (FITC-conjugated sheep anti-mouse IgG; Boehringer-Mannheim, Indianapolis, IN; Cat.
After overnight incubation, membranes were washed and incubated with peroxidase labeled secondary antibody (goat anti-mouse IgG and IgM, pre-absorbed with human serum and diluted 1: 2000; Jackson Immunoresearch, West Grove, PA) for 1 hour at room temperature.
After several washes in PBS (room temperature), the sections were incubated with peroxidase labeled secondary antibody - goat anti-mouse IgG and IgM, pre-absorbed with human serum (Jackson Immunoresearch, West Grove, PA), diluted 1:50 and absorbed with rat kidney homogenate as described [ 39 ] . Tissue sections were processed further as described previously [ 40 ] , including hematoxylin counterstaining of the nuclei.
One quarter of each fraction was subjected to SDS-PAGE and immunoblot analysis using the 9E10 monoclonal antibody against the myc epitope (Oncogene Research Products, Cambridge, MA), followed by HRP-conjugated goat anti-mouse IgG and ECL detection reagent (Amersham Corp, Arlington Hts.
Antibodies used: rabbit Arp3 antibodies (gift from C. Egile using the same peptide as described [ 33 ] ) used at 1:500 dilution, mouse actin antibodies (Santa Cruz SC-8432) used at 1:10,000 dilution, rabbit VASP antibodies (gift from F. Southwick) used at 1:100, rabbit WAVE1 antibodies (gift from S. Eden as described [ 26 ] ) used at 1:100 and Alexa-dye labeled secondary antibodies, goat anti-rabbit and goat anti-mouse (Molecular Probes).
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