Words similar to anti-mouse
Example sentences for: anti-mouse
How can you use “anti-mouse” in a sentence? Here are some example sentences to help you improve your vocabulary:
For IP 3 R3 double-labeled sections, Cy-5 anti-mouse secondary antibodies were applied.
After several washes in PBS (room temperature), the sections were incubated with peroxidase labeled secondary antibody - goat anti-mouse IgG and IgM, pre-absorbed with human serum (Jackson Immunoresearch, West Grove, PA), diluted 1:50 and absorbed with rat kidney homogenate as described [ 39 ] . Tissue sections were processed further as described previously [ 40 ] , including hematoxylin counterstaining of the nuclei.
After washing three times, cells were then incubated in FITC-conjugated anti-mouse secondary antibody (1 : 400) dilution for 1 h at 37°C, followed by washing three times with anti-fade equilibrating buffer and mounting in anti-fade mounting medium (Molecular Probe, Eugene, OR, USA).
We used an anti-mouse CD31 antibody (American Diagnostica), that cross reacts with rat CD31, to stain specifically the endothelial cells that can then be easily selected by the image analyser software Qwin™.
After blocking non-specific binding sites with 3% BSA, 0.1% tween-20 in PBS, the coverslips were incubated with anti-HA (40 μg/ml) or anti-GFP antibody (40 μg/ml), rabbit anti-mouse IgG (Pierce, 1:200), biotin-conjugated anti-rabbit IgG (Vector Laboratories, 1:200), and streptavidin conjugated with either Alexa 488 or Alexa 594 (Molecular Probes, 1:200).