Words similar to anti-human
Example sentences for: anti-human
How can you use “anti-human” in a sentence? Here are some example sentences to help you improve your vocabulary:
Blots were first incubated overnight with gentle shaking at 4°C with rabbit anti-human TRAIL polyclonal antibodies (Cell Sciences, Inc., Norwood, MA) at a concentration of 0.2 μg/ml in Buffer A. Membranes were then washed the following day 3 times for 5 min in Buffer B with gentle shaking, then incubated for 1 h with gentle shaking at room temperature with horseradish peroxidase (HRP)-conjugated anti-rabbit immunoglobulin G (Amersham Biosciences Corp., Piscataway, NJ) diluted 1:5000 in Buffer A. Reactive proteins were visualized by enhanced chemiluminescence (Amersham, Arlington Heights, IL) following exposure to X-ray film and subsequent film development.
The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
TRAIL may mediate a number of physiological responses; therefore, in order to determine if TRAIL expressed by S. aureus -infected osteoblasts mediates osteoblast apoptosis, cultured normal mouse osteoblasts were infected with S. aureus strain UAMS-1 at various MOIs in the presence and absence of an anti-human TRAIL polyclonal antibody.
211), mouse anti-human CCR5 mAb (45531.
Peripheral blood mononuclear cells (PBMCs) were incubated overnight at 50,000 to 100,000 cells/well in 96-well polyvinylidene plates that had been precoated with 0.5 μg/ml anti-human interferon-γ monoclonal antibody (Mabtech, Stockholm, Sweden).
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