Example sentences for: anti-goat

How can you use “anti-goat” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Blots were washed twice with TBS-T containing 0.5% milk, then incubated at room temperature for 2 hours in TBS-T containing 0.5% milk and 1:5000 dilutions of secondary Ab conjugated to peroxidase (for BMP-5, anti-goat IgG-peroxidase from Chemicon, Temecula CA; for Smad1, anti-rabbit Ig-peroxidase from Amersham, Piscataway, NJ).

  • Staining then proceeded in standard fashion using goat polyclonal anti-FasL antibody (goat polyclonal antibody IgG raised against FasL(N-20), Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1-4 μg/ml, followed by biotinylated donkey anti-goat IgG.

  • After extensive washing, the slides were incubated with biotinated donkey anti-goat polyclonal antibody (DAKO) pre-absorbed with rat kidney homogenate [ 15 ] , followed by peroxidase-coupled streptavidin, and the peroxidase visualized by diaminobenzidine as recommended by the kit (LSAB) vendor (DAKO).

  • Panel D shows control staining with PBS and anti-goat secondary antibody.

  • The following fluorochrome-labeled antibodies were used (AlexaFluor, Molecular Probes): goat anti-mouse IgG (H+L) conjugate (#A-11032; red), goat anti-rabbit IgG (H+L) conjugate (#A-11046; blue), and donkey anti-goat IgG (H+L) conjugate (#A-11055; green).

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