Example sentences for: anti-digoxigenin

How can you use “anti-digoxigenin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The labelled probe molecules were detected with rhodamine anti-digoxigenin (Oncor, Inc., Gaithersburg, MD) and the slides were washed 3 times in PBS and 0.1 % Nonidet NP-40.

  • Slides were equilibrated for 5 min in TBS pH 7.5 (100 mM Tris, 150 mM NaCl), and then incubated for 2 h with alkaline phosphate-conjugated anti-digoxigenin antibody (Roche, Cat No.

  • After incubation with avidin or anti-digoxigenin, slides were washed in coplin jars on a rotating shaker for three 5-min washes in 4x SSC/0.

  • In single-color hybridization experiments, biotinylated or digoxigenin-labeled BAC probes were detected using FITC-avidin (Vector Laboratories) or FITC anti-digoxigenin (Roche), respectively.

  • Sections were then incubated for 2 hours at room temperature with alkaline phosphatase-coupled anti-digoxigenin antibody (Boehringer Mannheim, Indianapolis, IN) diluted 1:5000 in Buffer 1 containing 0.5% Blocking reagent (Boehringer Mannheim).

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