Words similar to anti-cyclin
Example sentences for: anti-cyclin
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The following antibodies and dilutions were utilized: anti-neurofilament 270 (specific to ganglion cells and horizontal cells, 1:100) [ 74 ] , anti-cyclin D3 (Muller cells, 1:500, Santa Cruz Biotechnology), VC1.
Briefly, cellular protein extracts (25 μg) were precleared with 10 μl of 10% preimmune normal rabbit serum followed by immunoprecipitation with either 3 μg anti-cyclin D1, E polyclonal antibodies (Upstate Biotechnology), 2 μg anti-cyclin A polyclonal antibody antibodies (C-19)-G (Pharmingen), or normal rabbit preimmune serum as a negative control.
7% formaldehyde at -20°C for 20 minutes, then washed twice with 1 × PBS; the coverslips where then incubated with a 1:200 dilution of rabbit anti-cyclin D 1 antibody (Santa Cruz Biotechnology) or control rabbit sera (Biogenex) for 2 hours at room temperature followed by three washes with 1 × PBS; the coverslips were then incubated with a 1:250 dilution of rhodamine-labeled donkey-anti-rabbit anti-sera (Chemicon) for 45 minutes to detect the primary antibody; following three final washes with 1 × PBS, the coverslips were mounted on glass slides using ProLong Antifade (Molecular Probes) for fluorescent detection of GFP and cyclin D 1 on an Olympus IX70 microscope at 100 × magnification.
The HE12 anti-cyclin E antibody was utilized as a positive control for Cdk2 IP.
Following 7.5% SDS/PAGE, podocyte protein (20 μg) was analyzed by western blot for cyclin D 1 (ECL detection of a 1:1000 dilution of rabbit anti-cyclin D 1 antibody, Santa Cruz BioTechnology).
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