Words similar to ani
Example sentences for: ani
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We did not detect similar fluctuation of nuclear p27 expression in muscle fibers, yet myotubes do not exhibit estrogen receptor expression [ 2 26 ] . However, estrogen, progesterone and androgen receptors are expressed in the principal cells (fibroblasts) of levator ani fascia [ 26 ] , which represents another important component of the pelvic floor involved in its suspensory function, and might be a target of the complex effect of hormonal fluctuations during the cycle, and influenced by the hormone replacement therapy in postmenopausal women.
7Ashows cumulative data on p27 expression in levator ani muscle from asymptomatic and symptomatic patients.
Additional pelvic floor structure, the levator ani fascia, was studied and exhibited nuclear LHR immunoreactivity in principal cells (fibroblasts; white arrowhead, Fig.
From the ovarian sex steroids, the role of diminishing estrogen levels is questionable, since the levator ani muscle fibers do not express estrogen receptor alpha [ 2 26 ] . It is not known if estrogen receptor beta is expressed, but levator ani muscle fibers show nuclear expression of progesterone and androgen receptors [ 26 ] . Altered ovarian function in the premenopause can be observed as early as age 43 years, and includes hyperestrogenism and hypergonadotropism, accompanied by decreased progesterone secretion [ 27 ] . During the transition from pre- to postmenopause both estrogen and androgen levels also decline [ 28 ] . These hormonal alterations may well be responsible for the increased gynecological morbidity that characterizes this period of life, including structural and molecular changes in levator ani muscle and increasing incidence of pelvic floor disorders.
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).
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