Words similar to amv
Example sentences for: amv
How can you use “amv” in a sentence? Here are some example sentences to help you improve your vocabulary:
To this, 4 μl of a 5×AMV RT reaction buffer, 2 μl of 25 mM MgCl, 2 μl of 10 mM dNTP, 0.5 μl of Rnasin (1 U/μl) (Promega), 1 μl of oligo(dT) 15 (0.
These oligonucleotides (100 pmole) were annealed and extended with AMV reverse transcriptase (Amersham Pharmacia Biotech, Piscataway, NJ; 45 units) in AMV RT buffer (50 mM Tris-HCl, pH 8.3, 8 mM MgCl 2 , 50 mM NaCl, 1 mM DTT) and dNTPS (200 μM) for 30 minutes at 37°C.
- AMV : Anterior medullary velum
3B); (iii) the DNA polymerase activity of the P survived the low pH treatment, as measured by synthesis of viral DNA; and (iv) the RNAseH substrates were suitable because they were degraded by E. coli RNAseH and AMV reverse transcriptase and because the DHBV RNAseH activity can degrade these RNA sequences during reverse transcription (the target RNAs were derived from the DHBV genome).
The RT reaction was performed using AMV reverse transcriptase, and all PCRs were performed with a 15:1 mixture of Taq:Pfu polymerase.
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