Words similar to ammonium
Example sentences for: ammonium
How can you use “ammonium” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.
Sample RNA and the antisense probe were mixed and co-precipitated with 0.5 M ammonium acetate.
The samples were applied to the columns and eluted with 0.1 M ammonium bicarbonate containing 0.1% Triton X-100 (Pierce, Rockford, Illinois, USA).
The ERCA mix contained 5% tetramethyl ammonium oxalate [ 21 ] , 400 μM dNTP mix, 1 uM each of the two primers, 8 u of Bst polymerase (New England Biolabs, MA), and 1X modified ThermoPol reaction buffer containing 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH 4 ) 2 SO 4 and 0.1% Triton X-100.
After cooling, 9.1 ml of an aqueous ammonium molybdate solution (26% by weight) and 0.4 ml of Fiske-Subbarow reagent were added and the samples were then placed in a boiling water bath for 10 min.