Example sentences for: ammonium

How can you use “ammonium” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After concanavalin A-Sepharose 4B and nickel affinity column chromatography, the ammonium sulfate precipitated sCD39 was collected and resuspended in about 50 μl of 40 mM Tris-HCl (pH7.

  • Crystals were grown by the hanging drop method in which 2 μl of the 7.5 mg/ml dimer solution in 10 mM bis tris propane (pH 8.5) and 200 mM NaCl, were mixed with 2 μl of reservoir solution containing 5% PEG 4000 and 2.0 M ammonium sulfate.

  • Histone samples were resolved by electrophoresis using 15% acid-urea gel containing 36%w/v urea, 5%v/v acetic acid, 600 μl TEMED and 0.7 ml of 10% ammonium persulfate prepared as described elsewhere [ 27].

  • The serine/threonine protein phosphatase type 5 (PP5) was purified from bovine brain according to the following procedure: a bovine brain was homogenized in ~ 3 volumes of buffer A (50 mM HEPES pH 7.5 4°C, 1 mM EDTA, 10 mM PMSF and 0.1% 2-mercaptoethanol) and subjected to centrifugation at 15,000 g for 40 min. followed by a 45%-60% ammonium sulfate fractionation of the supernatant.

  • The resulting suspension was subjected to ammonium sulphate precipitation (starting with 10% followed by 30% and finally 50%).

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