Words similar to ammonium
Example sentences for: ammonium
How can you use “ammonium” in a sentence? Here are some example sentences to help you improve your vocabulary:
FLAG-MHCK-C was purified using a combination of ammonium sulfate fractionation and FLAG-peptide affinity chromatography.
SC medium is 1.7 g/l yeast nitrogen base without amino acids and ammonium sulfate, 5 g/l ammonium sulfate, 20 g/l glucose, 20 mg/l uracil, 20 mg/l adenine, 80 mg/l L-leucine, 20 mg/l L-histidine, 40 mg/l L-tryptophan, 20 mg/l L-methionine, and 30 mg/l L-lysine.
The gel core was diced into small pieces, destained with 1:1 v/v 30 mM potassium ferricyanide and 100 mM sodium thiosulfate, reduced in 10 mM DTT, and alkylated in 50 mM iodoacetamide in 0.1 M ammonium bicarbonate.
The serine/threonine protein phosphatase type 5 (PP5) was purified from bovine brain according to the following procedure: a bovine brain was homogenized in ~ 3 volumes of buffer A (50 mM HEPES pH 7.5 4°C, 1 mM EDTA, 10 mM PMSF and 0.1% 2-mercaptoethanol) and subjected to centrifugation at 15,000 g for 40 min. followed by a 45%-60% ammonium sulfate fractionation of the supernatant.
Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .
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