Example sentences for: ammonium

How can you use “ammonium” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.

  • The 20/25% ammonium sulfate protocol seems to give a better purification of proteins that can be precipitated at 25%, but the alternative protocol is important for some mutants that do not precipitate completely at 25% ammonium sulfate.

  • The ammonium sulfate precipitate, containing the FLAG-MHCK-C protein, was collected by centrifugation and resuspended with gentle douncing in 20 ml TBS containing 1 mM EDTA (TBSE), and 1X PIC1 and PIC2.

  • The sections were placed in 2% cobalt chloride solution for 3 minutes, washed in four 30-sec changes in 100 mM 2-amino-2-methyl-1-propanol at pH 9.4, developed in 1% ammonium sulfide solution for 3 minutes, washed in running tap water for 5 minutes, dehydrated in graded ethanol, cleared in xylene and mounted in Canada balsam.

  • An additional 1 ml of ammonium acetate was added to the pellet, the homogenization was repeated, the contents were centrifuged and the supernatant was collected.


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