Example sentences for: ammonium

How can you use “ammonium” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The pellets from the ammonium sulfate fractionation were resuspended in 60 ml of buffer B (20 mM HEPES pH 8.5 4°C, 1 mM EDTA, and 0.1% 2-mercaptoethanol), desalted on a G-25 sephadex column equilibrated with buffer B, then subjected to sequential chromatography on a 5 ml HiTrap Heparin column (Pharmacia), a 5 ml HiTrap Q column (Pharmacia) and a HiTrap SP column (Pharmacia) respectively.

  • The supernatant fractions were pooled, passed through a pre-cycled CM-52 column and eluted with a gradient of 0.01 M to 0.5 M ammonium acetate (pH 6.4) at a flow rate of 3 ml/h using a Pharmacia peristaltic pump.

  • Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .

  • FtsZ was precipitated by adding ammonium sulfate to 35% saturation.

  • A sector containing XLD (a selective media for Salmonella comprised of xylose, lysine, sodium desoxycholate, sodium thiosulfate, and ferric ammonium citrate) (Difco, Detroit, MI) was also included as an additional phenotypic test.


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