Words similar to amersham
Example sentences for: amersham
How can you use “amersham” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, equal nuclear cell extract (200 μg/sample) was mixed with 3 μg anti-E2F-4 antibodies at 4°C followed by the addition of 50 μl protein A-sepharose beads (Amersham Biotechnology).
Multiple tissue Northern blots containing 2 μg poly (A) + RNA (Human Multiple Tissue Northern Blot IV and Human Multiple Tissue Northern Blot; Catalog numbers: 7766-1 and 7760-1; CLONTECH) were hybridized with 32P-labeled human cDNA probes for TOP1(nucleotides 212-1661, GenBank accession number J03250), BTBD1 (nucleotides 84-3196, GenBank accession number AF355402) or BTBD2 (nucleotides 1229-2668, GenBank accession number AF355797) in Rapid-hyb buffers (Amersham Life Science, RPN1635).
Subsequently, blots were washed three times as described above, and visualized using an enhanced chemiluminescence detection method (ECL, Amersham).
Briefly, the reaction was started by adding 3 μl of 10 mM GTP (Sigma) and 5 μCi {α- 32P} GTP (3000 Ci/mmol, Amersham) to 27 μl of FtsZ in MEMK6.
Duplicates of membrane preparations equivalent to 0.1 mg protein were incubated with 125I-EGF (50 or 100 ng; Amersham Life Sciences, Arlington Heights, IL) in 200 μl reaction buffer (5 mM Tris-HCl, pH 7.0, 125 mM sucrose, 75 mM NaCl, 0.5 mM Ca 2+and 0.5% BSA) at 25°C for 60 min.
Loading...