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Example sentences for: amersham

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To generate the probe of HL60 DNA, a PCR reaction was set up with 0.5 μl of the reassociated DNA, 5 μl of 10x Clontech PCR buffer, 1 μl of dNTPS (10 mM dATP, dTTP, and dGTP each, 6 mM dCTP), 4 μl of FluoroLink Cy3-dCTP (Amersham Pharmacia), 4.5 μl of 10 μM CT primer, 1 μl of Clontech cDNA polymerase and 34 μl of ddH 2 O. In the PCR reaction for the probe of HL60/TLK286 DNA, Cy5-dCTP and TT primer were used instead of Cy3-dCTP and CT primer.
All PCR products were analyzed on agarose gels, and reactions with no detectable product, multiple bands or bands of unexpected size were repeated using Herculase (Amersham) or Expand polymerase (Roche).
Bound antibodies were detected by a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer's protocol.
After four additional washes with 0.1% Tween/TBS, bound primary antibodies were visualized using an ECL detection system and recorded on radiographic film (Amersham Pharmacia Biotech).
Proteins were detected using an Enhanced Chemi-Luminescence (ECL) detection system (Amersham Life Sciences, Arlington Heights, IL, USA).