Example sentences for: amersham

How can you use “amersham” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, equal nuclear cell extract (200 μg/sample) was mixed with 3 μg anti-E2F-4 antibodies at 4°C followed by the addition of 50 μl protein A-sepharose beads (Amersham Biotechnology).

  • Multiple tissue Northern blots containing 2 μg poly (A) + RNA (Human Multiple Tissue Northern Blot IV and Human Multiple Tissue Northern Blot; Catalog numbers: 7766-1 and 7760-1; CLONTECH) were hybridized with 32P-labeled human cDNA probes for TOP1(nucleotides 212-1661, GenBank accession number J03250), BTBD1 (nucleotides 84-3196, GenBank accession number AF355402) or BTBD2 (nucleotides 1229-2668, GenBank accession number AF355797) in Rapid-hyb buffers (Amersham Life Science, RPN1635).

  • Subsequently, blots were washed three times as described above, and visualized using an enhanced chemiluminescence detection method (ECL, Amersham).

  • Briefly, the reaction was started by adding 3 μl of 10 mM GTP (Sigma) and 5 μCi {α- 32P} GTP (3000 Ci/mmol, Amersham) to 27 μl of FtsZ in MEMK6.

  • Duplicates of membrane preparations equivalent to 0.1 mg protein were incubated with 125I-EGF (50 or 100 ng; Amersham Life Sciences, Arlington Heights, IL) in 200 μl reaction buffer (5 mM Tris-HCl, pH 7.0, 125 mM sucrose, 75 mM NaCl, 0.5 mM Ca 2+and 0.5% BSA) at 25°C for 60 min.


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