Example sentences for: alto

How can you use “alto” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Starting materials were the previously cloned genomic subclones and a plasmid carrying EGFP-F (pEGFP-F from Clontech, Palo Alto, CA).

  • Blots were prehybridized and hybridized at 42°C in ExpressHyb (Clontech, Palo Alto, CA) and washed in 0.1× SSC/1% SDS for one hour at 65°C.

  • The NYT reports that Louis Kao, owner of the Palo Alto Chinese restaurant that in the 1970s played host to a number of men who pioneered the Internet and the personal computer industry, yesterday cooked his last meal.

  • Human umbilical vein endothelial cell (EC) cultures were purchased (Clontech, Palo Alto, CA) and maintained at 30-80% confluency to ensure high viability.

  • A549 cells were grown to 50-80% confluence on 12-well plates and transfected with the Mercury pathway profiling system vector, pNFκB-TA-Luc (Clontech, Palo Alto, CA, USA), using lipofectamine (Gibco/BRL, Rockville, MD, USA) and used according to the manufacturer's recommendations.


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