Words similar to alexa

Example sentences for: alexa

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After blocking non-specific binding sites with 3% BSA, 0.1% tween-20 in PBS, the coverslips were incubated with anti-HA (40 μg/ml) or anti-GFP antibody (40 μg/ml), rabbit anti-mouse IgG (Pierce, 1:200), biotin-conjugated anti-rabbit IgG (Vector Laboratories, 1:200), and streptavidin conjugated with either Alexa 488 or Alexa 594 (Molecular Probes, 1:200).
Alexa 488-SP produced the most prominent increase in non-specific fluorescence in the absence of cells.
Also, the Alexa dyes are very stable fluorophores, even under UV light exposure, so that fading is unlikely to be a significant issue in influencing the results [ 34 ] .
When 1 nM or 10 nM Alexa 488-SP was added the fluorophore produced signals similar in intensity to those observed when this analog was added to transfected or untransfected CHO cells (data not shown).
Furthermore, Alexa 488-SP did not exhibit functional activity in two different assays.