Words similar to aldh
Example sentences for: aldh
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Spectrofluorometric assays for ALDH activity in HL-60 Cells
ALDH1A1 activity was measured on a Shimadzu UV1201 Spectrophotometer at 36.
In vitro, purified mouse [ 11 ] , human [ 12 ] , and Xenopus [ 13 ] ALDH1A1 can efficiently oxidize all-trans retinal to all-trans retinoic acid.
Previous reports of HPLC assays for ALDH reactions with tissue extracts used a single time point at 60 min to measure accumulation of product, after documenting that retinoic acid formation was still linear in time course experiments [ 33 ] . In our hands, the reaction with purified enzyme was only linear for product formation up to 10-15 minutes.
36 μM) as substrates, with the human ALDH1A1 showing the same inhibition with retinal or propanal as substrate.