Words similar to aldehyde
Example sentences for: aldehyde
How can you use “aldehyde” in a sentence? Here are some example sentences to help you improve your vocabulary:
In one report, ALDH1A1 protein was not detected by Western blot, but enzyme activity was detected using flow cytometry and a fluorescent aldehyde substrate [ 5 ] . In a second study, ALDH1A1 protein was detected using an ELISA assay and activity was detected using aldophosphamide as substrate [ 31 ] . In these two studies, designed to look at the relationship of ALDH1A1 activity to antitumor drug resistance, the levels of ALDH1A1 were low in HL-60 compared to other cell types, corresponding to the sensitivity of HL-60 cells to oxazaphosphorines.
The order of addition in the assay was as follows: buffer; pyrazole; NAD; ALDH (10 mU mouse liver ALDH1A1; 5 mU human erythrocyte ALDH1A1; or HL-60 cell extract); inhibitor (if any); and lastly, the aldehyde substrate.
The reaction was started by the addition of a cocktail of 8 μM ubiquitin, ATP regenerating system (2 mM HEPES at pH 7.4, 1 mM ATP, 30 mM creatine phosphate, 1 mM magnesium acetate, 0.15 mg/ml creatine kinase), reaction buffer (4 mM magnesium acetate, 1 mM DTT), 500 nM bacterially expressed 6xHis-UBCs, 100 nM 6xHis-tagged human E1, and 0.5 μM ubiquitin aldehyde in a volume of 15 μl.
To observe the microtubule cytoskeletons of rad26Δ and rad3Δ , cells were processed for immunofluorescence microscopy using standard combined aldehyde (formaldehyde and methanol) fixation and TAT1 monoclonal antibodies [ 65 ] .
Assay conditions were as given for the spectrophotometric enzyme assays above, with propanal or all-trans retinal used as the aldehyde substrate.
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