Example sentences for: alcian

How can you use “alcian” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Proliferation was assessed by cell counting, and maturation was ascertained visually as cartilage nodule formation, and histochemically by Alcian Blue staining.

  • Undigested hyaluronic acid is stained with Alcian blue and shows a dark blue background against an unstained area with digested hyaluronic acid.

  • For Alcian Blue staining, cells were fixed in 70% Ethanol for 30 minutes and then incubated for one hour with the Alcian Blue staining solution, which consisted of 15 mg Alcian Blue 8GX (Sigma) in 30 ml of 95% Ethanol and 20 ml Glacial acetic acid.

  • We used histochemical staining with Hematoxylin and Eosin to ascertain cell morphology; and Alcian Blue at pH 1 [ 43 ] to detect cartilage matrix sulfated glucosaminoglycans as described before [ 1 ] . The production of Collagen type II was assessed by immunohistochemistry using a monoclonal antibody against mouse type II Collagen (Chemicon), and secondary antibody and colorimetric reaction kit (Histostain Plus, Zymed).

  • They were rinsed with water and transferred to staining solution which contained 1 volume of 0.1% Alizarin red S (Sigma), 1 volume of 3% alcian blue (Sigma), 1 volume acetic acid and 17 volumes ethanol.


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