Example sentences for: alcian

How can you use “alcian” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We used histochemical staining with Hematoxylin and Eosin to ascertain cell morphology; and Alcian Blue at pH 1 [ 43 ] to detect cartilage matrix sulfated glucosaminoglycans as described before [ 1 ] . The production of Collagen type II was assessed by immunohistochemistry using a monoclonal antibody against mouse type II Collagen (Chemicon), and secondary antibody and colorimetric reaction kit (Histostain Plus, Zymed).

  • Cartilage was stained with alcian blue followed by bone staining with alizarin red.

  • Alcian Blue (Sigma) was used to detect extracellular matrix proteoglycans in chondrogenic cultures.

  • For Alcian Blue staining, cells were fixed in 70% Ethanol for 30 minutes and then incubated for one hour with the Alcian Blue staining solution, which consisted of 15 mg Alcian Blue 8GX (Sigma) in 30 ml of 95% Ethanol and 20 ml Glacial acetic acid.

  • After 28 d in culture, more than 50% of all cells exhibited robust staining for Alcian Blue, a marker specific for extracellular matrix proteoglycans.

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