Words similar to alameda
Example sentences for: alameda
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Oligonucleotides were synthesized at a 50 nmol scale by Invitrogen/Life Technologies (Carlsbad, CA) or Operon Technologies (Alameda, CA), and resuspended in sterile milliQ water to a final concentration of 100 μM.
Oligonucleotide primers for PCR were synthesized by Operon Technologies (Alameda, CA, USA).
Decamers from kits B, F, G and AP (Operon Technologies Inc., Alameda, California, USA) were used as primers.
The resulting cDNA was used as template for PCR amplification using the primer pairs (Operon Technologies, Alameda, CA, USA) listed in Table 1. As a loading control parallel PCR reactions were carried out using a primer pair for human GAPDH (see Table 1).
Forty microliters of the amplified products was also independently tested for HPV using a reverse line probe assay (typing strip and reagents generously provided by Janet Kornegay, Roche Molecular Systems, Inc., Alameda, CA).
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