Example sentences for: agarose

How can you use “agarose” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After clarification by centrifugation at 12,000 × g for 10 min, the cell extract was incubated with T7 tag antibody (Ab)-agarose beads (Novagen) for 2 h.

  • The amplified 169-base-pair fragment was digested with 0.4 U of the restriction enzyme Mnl I (New England Bio Labs, Beverly, MA) at 37°C for 6-12 hours and the resulting fragments were subjected to electrophoresis on 4% Nu-Sieve GTG agarose gels (FMC Bioproducts, Rockland, ME) and the nucleotide bands visualized by ethidium bromide fluorescence and photography.

  • H441 cells were harvested and total RNA isolated as described previously [ 16 ] . Equal amounts of total RNA from each condition were separated on agarose gels, transferred to Nytran-plus membranes (Schleicher and Schuell, Keene, NH, USA), and subsequently hybridized with a radiolabeled human SP-A cDNA.

  • RNA was fractionated via denaturing agarose gel electrophoresis using glyoxal according to standard protocols [ 76 ] . Immediately before use the glyoxal was deionized by vortexing in the presence of mixed bed resin beads (Sigma Chemical Co.

  • PCR products were resolved by electrophoresis on 1.5% agarose gels and identified with ethidium bromide staining.


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