Example sentences for: agarose

How can you use “agarose” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Purified MSP products were sequenced in representative specimens by direct sequencing to confirm the methylation identified on agarose gels.

  • Game and his colleagues have developed a sensitive means of detecting chromosomal ds breakage using a circular chromosome [ 3 ] . Under most conditions of pulsed-field gel electrophoresis, a circular yeast chromosome and circular bacterial chromosomes will not enter the gel, very likely because they are trapped by the branches of the network of agarose [ 3 13 14 ] . One double-strand break transforms this circular form into a linear form, which can now move slowly in the gel [ 3 ] . We used this procedure to detect double-strand breakage of a circular bacterial chromosome occurring spontaneously or after loss of a restriction-modification gene complex [ 15 16 ] . We found increased chromosome breakage in recBC -null and recC1002 mutants of E. coli under both conditions [ 1 ] . Michel and her colleagues used pulsed-field gel electrophoresis to detect degraded chromosomal DNAs arising spontaneously in recBC mutants and arising during replication fork arrest [ 2 ] . RuvABC proteins, which catalyze migration and cleavage of Holliday junctions, are responsible for the occurrence of the degraded DNAs following replication fork arrests [ 17 ] .

  • The products were separated and analyzed on a 3% agarose gel.

  • The resulting cDNA inserts were purified by agarose gel electrophoresis, digested with Eco R I and cloned into pBluescript KS- (Stratagene, La Jolla, CA).

  • Products were electrophoresed on 2% agarose gels and stained with ethidium bromide.


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