Words similar to aflp

Example sentences for: aflp

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Table 1lists the isolates tested, their genomic relatedness to B. anthracis as determined by Jaccard distances calculated from AFLP profile comparisons [ 17 18 ] , and the number of positive PCR reactions obtained for each isolate.
Enriching the AFLP or specific adaptor-amplified DNA fragments is a simple and efficient approach for SSR isolation and has been successfully applied to a number of plant genomes [ 12 28 29 30 31 32 ] . By employing the modified procedures from other plant species, corresponding isolating protocols were established in peanut for development of microsatellites through this study.
Although a considerable number of AFLP markers have been identified [ 27 ] , substantially more DNA markers are needed to saturate the existing peanut linkage map and to initiate genetic studies for this plant species.
By optimizing the hybridization and washing steps, most of the AFLP fragments were found to harbor microsatellites after cloning and sequencing.
Digested fragments were ligated with corresponding adapters and then amplified using one-base selective primers ( Hind III-A and Mse I-T) according to standard AFLP protocol [ 22 ] . Three biotinylated SSR probes, (AT) 15 , (GT) 15 , (GA) 15 , which were reported being rich in other plant species, were separately hybridized with amplified AFLPs.