Words similar to actomyosin
Example sentences for: actomyosin
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Identification of I and IIa fibers by immunohistochemistry was fully concordant with identification of these fiber types by the myofibrillar actomyosin ATPase histochemical staining procedure.
Two methods were used: (1) The histochemical method of Ogilvie and Feeback [ 18 ] , based on staining for myofibrillar actomyosin ATPase, and (2) immunohistochemistry based on the reaction of specific antibodies for mysosin heavy chain (MHC) isoforms [ 34 35 ] . In the histochemical staining procedure, sections were pre-incubated in (0.
[ 48 ] have shown a major difference in actomyosin ATPase activity in rat single IIb and IIx fibers, and Sieck et al.
Non random error (confounding) was also considered, including fiber size differences between regions and the number of nuclei visible in myofibrillar actomyosin ATPase slide preparations between regions (see Figure 1C).
The procedure for the histochemical demonstration of actomyosin ATPase was employed as described by Goth and Samara [ 24 ] . Briefly, fixed sections were washed in rinse solution [18 mM calcium chloride in 100 mM tris (hydroxymethyl) aminomethane (Tris), pH 7.8] for 1 minute, preincubated either in 50 mM potassium acetate in 18 mM calcium chloride at pH 4.35 (for acid-stable myosin ATPase, type I slow twitch muscle fibers), or in 18 mM calcium chloride in 100 mM 2-amino-2-methyl-1-propanol at pH 10.