Words similar to actb
Example sentences for: actb
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A semi-quantitative analysis of gene expression was performed in replicate experiments using 26-28 cycles of multiplex RT-PCR with β-actin ( ACTB ) as control and gene specific primers spanning at least 2 exons.
A semi-quantitative analysis of gene expression was performed using 26 to 28 cycles of multiplex RT-PCR with β-actin ( ACTB ) as control and gene specific primers spanning at least 2 exons, except in RASSF1A . For the latter, we used single PCR with primers and conditions as previously described [ 16 ] . The gene primers used and their positions in respective cDNAs were:
To examine the biological role of promoter methylation in CC, we assessed the levels of mRNA by semi-quantitative RT-PCR in eight cell lines and compared with the respective control values obtained from the averages calculated from 2 normal cervix after normalization against ACTB . This analysis was performed on 7 genes ( CDH1 , DAPK , RARB , HIC1 , MGMT , RASSF1A , and MLH1 ) that exhibited methylation in one or more cell lines and primary tumors (Fig.
Levels of expression of each gene were assessed by comparing with the respective control values, obtained from the averages calculated from 2 to 4 normal testes, after normalization against ACTB . All tumors with promoter hypermethylation of the MGMT and MLH1 genes exhibited an absence or down-regulated expression of the respective gene, while 8 of 10 cases with RASSF1A methylation and 3 of 5 tumors with RARB methylation showed down-regulated expression (Table 1).
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