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Example sentences for: actb
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A semi-quantitative analysis of gene expression was performed using 26 to 28 cycles of multiplex RT-PCR with β-actin ( ACTB ) as control and gene specific primers spanning at least 2 exons, except in RASSF1A . For the latter, we used single PCR with primers and conditions as previously described [ 16 ] . The gene primers used and their positions in respective cDNAs were:
A semi-quantitative analysis of gene expression was performed in replicate experiments using 26-28 cycles of multiplex RT-PCR with β-actin ( ACTB ) as control and gene specific primers spanning at least 2 exons.
Levels of expression of each gene were assessed by comparing with the respective control values, obtained from the averages calculated from 2 to 4 normal testes, after normalization against ACTB . All tumors with promoter hypermethylation of the MGMT and MLH1 genes exhibited an absence or down-regulated expression of the respective gene, while 8 of 10 cases with RASSF1A methylation and 3 of 5 tumors with RARB methylation showed down-regulated expression (Table 1).
To examine the biological role of promoter methylation in CC, we assessed the levels of mRNA by semi-quantitative RT-PCR in eight cell lines and compared with the respective control values obtained from the averages calculated from 2 normal cervix after normalization against ACTB . This analysis was performed on 7 genes ( CDH1 , DAPK , RARB , HIC1 , MGMT , RASSF1A , and MLH1 ) that exhibited methylation in one or more cell lines and primary tumors (Fig.
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