Words similar to acridine
Example sentences for: acridine
How can you use “acridine” in a sentence? Here are some example sentences to help you improve your vocabulary:
For acridine orange staining, discs were dissected in Schneider's medium, incubated for 5 minutes in medium containing 1.6 × 10 -7M acridine orange, washed briefly and put on a slide in a drop of medium.
Fixed preparations were dropped onto clean glass slides and stained with 0.1 mg/ml acridine orange for 5 minutes, followed by a wash in running water for 2 minutes and a 1 minute incubation in Sorensen's buffer (67 mM KH 2 PO 4 + 67 mM Na 2 HPO 4 ). The wet slides were covered with glass coverslips and metaphases photographed immediately with a Zeiss Axioskop fluorescence microscope using the FITC filter and a 100× objective.
The slides were stained with acridine orange before microscopic evaluation.
Briefly, cell suspensions (100 μl) were mixed with acridine orange (5 μg/ml) and from this mixture an aliquot of 25 μl was dropped on to a microscope slide.
Hydrogen peroxide (50 μM) elicited a marked increase in apoptosis in AGS (Figure 4) and IEC-18 cells (Table 1), confirmed by acridine orange morphological assessment.