Example sentences for: absorbance

How can you use “absorbance” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Quantification of the relative amount of rhod-2 in the heart using absorbance measurements, was done by taking the ratio of absorbance at 524 nm (rhod-2 sensitive) to 589 nm (rhod-2 insensitive) which eliminated the effect of motion as both wavelengths would be equally affected by motion, though only 524 reflected the concentration of rhod-2 [ 7 ] . These wavelengths (524, 589 nm) were chosen as these were isosbestic points not effected by changes in absorbance of myoglobin induced by oxygen desaturation [ 7 ] . In solution maximal rhod-2 absorbance is at 554 nm.

  • Serial dilutions of the proteases were made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl 2 . Enzyme activity was measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA (Bachem Bioscience, Torrance, CA), 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer.

  • 4, along with previously published time traces at 600 nm [ 1 ] : the resting state (pR), M, N and O. The resting state, with an absorbance maximum of 546 nm (see above), provides the baseline spectrum for the difference time courses reported.

  • ATP (100 uM) was added to initiate the reaction and absorbance at 360 nm was read every ~6 seconds for 20 minutes.

  • To address these questions, we used an analytical method developed in our laboratory and described in a previous study [ 24 ] . Briefly, the complex cRNA is digested with P1 nuclease and calf intestinal phosphatase and applied to a high performance liquid chromatography (HPLC) column to separate the nucleosides, followed by an absorbance measurement at 260 nm.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast