Example sentences for: absorbance

How can you use “absorbance” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The absorbance, A , at 460 nm was read against a blank containing 2.0 mL of HSA stock plus 4.0 μL DMSO, and the [UCB] calculated using the extinction coefficient, ε, 47,000 M -1.cm -1 [ 18 ] . The pH measurement of each final DMSO/aqueous buffer system included an electrode calibration using the strong acid, HClO 4 [ 8 ] . The 0.1 M phosphate buffer in N = 0.31 DMSO was centrifuged because of partial insolubility [ 8 ] and only the supernatant was used.

  • The absorbance of contaminant proteins was assumed to be 1.1 for a 1 mg/mL solution.

  • The absorbance was measured at 410 nm using a microplate reader (Emax, Sunnyvale, CA).

  • Individual AV DNA titers were determined by three different methods: 1) plaque titration on 293 cells, 2) immunofluorescence microscopy of AV protein expression (anti-penton group antigen, clone 143, Biodesign, Kennebunk, ME) in 293 cells infected with serial dilutions of AV and 3) absorbance at 260 nm (pfu/ml = A

  • Quantification of the relative amount of rhod-2 in the heart using absorbance measurements, was done by taking the ratio of absorbance at 524 nm (rhod-2 sensitive) to 589 nm (rhod-2 insensitive) which eliminated the effect of motion as both wavelengths would be equally affected by motion, though only 524 reflected the concentration of rhod-2 [ 7 ] . These wavelengths (524, 589 nm) were chosen as these were isosbestic points not effected by changes in absorbance of myoglobin induced by oxygen desaturation [ 7 ] . In solution maximal rhod-2 absorbance is at 554 nm.


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