Example sentences for: aac

How can you use “aac” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For NRG1, the oligos used were HZ034, 5' TCG ACC AGC ATA TTA CTA CCC TTC GCA AAC TTT CAG GCA CTG TGC GGT ATT TCA CAC CG 3'; and HZ035, 5' GTA GTA CTG CTA ATG AGA AAA ACA CGG GTA TAC CGT CAA AGA TTG TAC TGA GAG TGC AC 3'.

  • All fusions were made in the GFP expression vector pTX-GFP [ 36 ] . The construct for MHCK A has been described previously [ 23 ] . Protein sequences for MHCK A, MHCK B, and MHCK C correspond to GenBank entries A55532, AAB50136, and AAC31918, respectively.

  • For insertion, two complementary pre-kinased 18-mer synthetic DNA oligos having the following sequences: 5'-ATA AAC AAT GAG ACA ATA-3 (sense strand) and 3'-TAT TTG TTA CTC TGT TAT-5' (antisense strand) were hybridized.

  • The residues in the MIDAS motifs lie on three closely apposed loops located on the upper edge of the β-sheet, where they form the metal-binding site, with oxygen atoms in the aspartate, serine and threonine residues coordinating the metal ion [ 9 ] . In integrin α2β1, which is a collagen receptor, the metal-binding site also binds the collagen ligand through the conserved glutamate in a GFOGER motif (O = hydroxyproline) completing the coordination sphere of the metal [ 28 ] . In vivo, the binding of collagen at this site appears to be regulated through a conformational shift in which the loops forming the MIDAS site change from a closed to an open conformation [ 29 30 31 ] . Other proteins containing a domain with all three MIDAS motifs include the D-subunit of magnesium chelatase [ 32 ] , Ca-activated chloride-channel protein [ 33 34 ] , nitrate reductase (accession AAC79447), and the D subunit of nitric oxide reductase (accession AAC45374).

  • The primer pair for β-actin was GTC CTC TCC CAA GTC CAC ACA (forward) and CTG GTC TCA AGT CAG TGT ACA GGT AA (reverse), that of IL-8 was CTG CGC CAA CAC AGA AAT TA (forward) and ATT GCA TCT GGC AAC CCT AC (reverse), that of MCP-1 was GCC TCC AGC ATG AAA GTC TC (forward) and TAA AAC AGG GTG TCT GGG GA (reverse), that of IP-10 was CCA CGT GTT GAG ATC ATT GC (forward) and TGG AAG ATG GGA AAG GTG AG (reverse), that of RANTES was CGC TGT CAT CCT CAT TGC TA (forward) and GCT GTC TCG AAC TCC TGA CC (reverse), that of MIP-1α was TGC AAC CAG TTC TCT GCA TC (forward) and ACA GGG GAA CTC TCA GAG CA (reverse), and that of MIP-1β was CTG GGT CCA GGA GTA CGT GT (forward) and ACA GTG GAC CAT CCC CAT AG (reverse).


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