Example sentences for: a-s
How can you use “a-s” in a sentence? Here are some example sentences to help you improve your vocabulary:
Rlk was immunoprecipitated with affinity purified anti-Rlk antibody for 3 hours at 4°C and immune complexes were recovered with Protein A-Sepharose, washed as described above, and boiled in SDS-sample buffer.
Indeed, the earlier Anglo-Saxons had little more luck with the voodoo h ; in a brief article in an 1888 issue of Notes and Queries , Prof. Skeat wrote: Only last week, I found ors for hors horse in an unedited A-S manuscript.
The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).
For immunoprecipitation, the lysates were incubated for 4 h at 4°C with 1 μg of antibody (all from Santa Cruz) pre-adsorbed on Protein A-Sepharose beads (Pharmacia).
After concanavalin A-Sepharose 4B and nickel affinity column chromatography, the ammonium sulfate precipitated sCD39 was collected and resuspended in about 50 μl of 40 mM Tris-HCl (pH7.
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