Words similar to a-myc

Example sentences for: a-myc

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To detect surface expression of the mGluR5a-myc construct, a cy3-conjugated, mouse anti-myc antibody (Sigma) was applied to live cells at room temperature for 20 minutes at a 1:150 dilution.
Figure 3A( upper ) illustrates a cell injected with cDNA encoding mGluR5a-myc alone (and coexpressing pEGFP as a positive marker for expression).
The latter hypothesis predicts a reduction in mGluR cell surface expression or perhaps a change in surface distribution in the presence of Siah1a, such as occurs in the presence of Homer proteins [ 24 44 45 ] . To test whether Siah1a expression altered the surface expression of group I mGluRs, the surface distribution of mGluR5a-myc was examined in the absence and presence of Siah1a.
Column 3 ("anti-myc cy3") shows surface labeling of the extracullular myc epitope on mGluR5a-myc using a cy3 fluorescently-conjugated primary antibody.
The mGluR5a-myc (pRK5; from P. F. Worley) has been described previously [ 24 ] . mGluR1a-GFP was constructed by inserting the mGluR1a cDNA into the pEGFP N1 vector (Clontech Laboratories, Palo Alto, CA).