Words similar to prlg
Example sentences for: prlg
How can you use “prlg” in a sentence? Here are some example sentences to help you improve your vocabulary:
In fact, all of the PrlA and PrlG suppressors are completely dependent on functional SecB for manifestation of suppressor activity [ 5 8 ] . Even certain proteins that are not normally SecB-dependent for translocation become SecB-dependent when they contain a signal sequence mutation and are exported via the suppressor pathway.
The PrlA (SecY) and PrlG (SecE) suppressors are compromised in their ability to proofread, allowing export of proteins with mutations, or even complete deletions, of the signal sequence.
In a wild type cell, the mutant precursor would be rejected at this point by SecY/E, but in a PrlA or PrlG suppressor strain, rejection would not occur and the mutant secretory protein would be exported.
The signal sequence is crucial for efficient translocation; mutations in the signal sequence significantly reduce export of the preprotein and complete deletions of the signal sequence eliminate essentially all export [ 5 6 7 8 ] . Selection for extragenic suppressors of such export defective preproteins led to the identification of the prl alleles of secY ( prlA ), secE ( prlG ), secA ( prlD ), and more recently secG ( prlH ) [ 6 9 10 11 ] . Early hypotheses predicted that the Prl suppressors function by expanded or altered interactions with signal sequences, facilitating recognition of mutant as well as wild type signal sequences [ 6 ] .
More recent observations support an alternative mechanism of action for the PrlA/SecY and PrlG/SecE suppressors [ 8 12 ] . First, PrlA/G-mediated suppression does not exhibit allele specificity; all prlA and all prlG alleles that have been examined suppress all signal sequence mutations, implying that suppression is not due to a specific altered interaction that allows recognition of a mutant signal sequence [ 8 12 ] . Furthermore, all of the prlA and prlG alleles suppress complete deletions of the signal sequence, suggesting that an interaction between the signal sequence and the Prl suppressor is not necessary for suppression or even for export [ 5 8 13 ] . Based on these observations, it was proposed that the PrlA and PrlG suppressors do not function through altered interactions with signal sequences, but rather by loss of recognition [ 8 12 ] . The wild type SecE and SecY proteins are thought to function in concert to proofread the signal sequence of secretory proteins, rejecting defective precursors from the export pathway.
Loading...