Words similar to pfpp

Example sentences for: pfpp

How can you use “pfpp” in a sentence? Here are some example sentences to help you improve your vocabulary:

These results suggest that PfPP1 plays an essential role in Plasmodium replication.
As mentioned earlier, inhibition studies using OA and calyculin A suggested the existence of a PP1-like activity in P. falciparum extracts [ 15 ] . However, our attempts to purify the native PfPP1 enzyme by chromatographic procedures resulted in only small amounts of activity, probably due to rapid inactivation during fractionation.
Growth and induction of E. coli BL21(DE3) containing pET-15b-PfPP1 and the RIG plasmid were carried out using procedures described earlier [ 14 48 ] , except that the culture was grown at 18°C in the presence of 2 mM MnCl 2 , and IPTG concentration was lowered to 0.4 mM.
RNA interference (RNAi), mediated by short interfering double-stranded RNA molecules (siRNA or dsRNA), is now recognized as a major mechanism of post-transcriptional gene silencing (PTGS) in essentially all eukaryotes [ 16 ] . Recently, the technique has been successfully applied to cultured mammalian cells, whereby introduction of 21-nucleotide long synthetic dsRNA molecules corresponding to specific mRNA sequences effectively and specifically degraded the cognate mRNAs and abrogated the expression of the corresponding proteins [ 17 18 ] . This prompted us to test a similar approach to knockdown PfPP1 function in the erythrocytic P. falciparum stages.
The use of recombinant PfPP1 and protein-protein interaction techniques should aid in characterizing these physiological regulatory subunits of PfPP1.