Words similar to pegfp-n

Example sentences for: pegfp-n

How can you use “pegfp-n” in a sentence? Here are some example sentences to help you improve your vocabulary:

pEGFP-N280 and pFLAG-N280 were generated by inserting a HindIII-StyI fragment (containing amino acid 70-350 of MmCOP1; StyI site was filled in by Klenow DNA polymerase) into pEGFP and pFLAG-CMV vectors digested by HindIII and SmaI.
005 μg μl -1; pEGFP-N1; Clontech) to facilitate later identification of successfully injected cells.
pEGFP-N280 was digested with EcoRI and BamHI, filled in with Klenow DNA polymerase on both ends, and re-ligated to generate pEGFP-N200.
The coding regions were tagged with a C -terminal green fluorescent protein (GFP) sequence by subcloning into the EcoR 1 site of the pEGFP-N1 vector (Clontech, Palo Alto, CA).
α i2 (1-10)-GFP was constructed by annealing the complementary oligonucleotides 5'-ctagcaccatgggctgcaccgtgtcggccgaggacaag-3' and 5'-ccggcttgtcctcggacacggtgcagcccatggtg-3' (corresponding to amino acids 1-10 of α i2 ) and ligating into AgeI-NheI digested pEGFP-N1.