Example sentences for: pbst

How can you use “pbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5 × 10 6viral particles in 50 μl PBST per well) and incubated with shaking on a rotary shaker at 150 nm at 25°C for 30 min.

  • Neutralite avidin (Southern Biotechnology Associates) (25 μg in 50 μl PBST per spot) was added to the spot areas within plastic rings, which had been treated with sulfo-NHS-LC-biotin, and allowed to bind to biotin moieties on the well surface.

  • Biotinylated Ad5.CMV-LacZ (1 × 10 7viral particles per spot), prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, were incubated in the spot areas within plastic rings at 25°C for 2 h, followed by washing the wells with PBST to remove unbound viral particles.

  • Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.

  • The viral particles were subjected to three rounds of ultrafiltration with ZM-500 centrifugal filtration units (molecular mass cut-off, 500 kDa; Millipore) using PBS (pH 7.4) containing 0.05% Tween 20 (PBST) as a diluent to remove non-virion-associated biotinylation reagent.


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