Example sentences for: overnight

How can you use “overnight” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were rinsed after overnight incubation with the DNA, and the medium was changed the next day.

  • The tissue was washed four times for 10 min in 2 ml Hank's balanced salt solution on a platform shaker, then fixed overnight in 4% paraformaldehyde.

  • poolside at the Pearl Continental Hotel in downtown Rawalpindi, running around Gilgit in disputed Kashmir Province and fending off aggressive beggars on the overnight sleeper train to Karachi.

  • Rabbit anti-phospho Erk MAPK (1:5000, Promega, Madison, Wl), rabbit anti-phospho JNK MAPK (1:5000, Promega, Madison Wl), rabbit anti-phospho p38 MAPK (1:1000, Cell Signaling, Beverly, MA), mouse anti-total Erk (1:5000, Zymed, San Francisco, CA) rabbit anti-total JNK MAPK (1:5000, Santa Cruz BioTechnologies, Santa Cruz, CA) or rabbit anti-total p38 MAPK (1:1000, Cell Signaling, Beverly, MA) were incubated with blots overnight (4°C).

  • Another (identical) membrane (second membrane) was incubated overnight (4°C) for ERα (clone h-151, 0.1 μg/ml) in blocking reagent.

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