Words similar to immunoblotted
Example sentences for: immunoblotted
How can you use “immunoblotted” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were stimulated with increasing concentrations of IGF-I, and whole cell lysates immunoblotted for tyrosine-phosphorylated proteins.
Thirty micrograms of cytoplasmic lysates or 20 μg nuclear lysates were loaded onto 12% SDS-PAGE gels and immunoblotted using chemiluminescent antibodies.
Equal volumes of each sample (cytosolic and cytoskeleton-associated components) were subjected to SDS-PAGE, transferred to a nitrocellulose membrane (Bio-Rad) and immunoblotted with primary antibody to β-actin and detected using horseradish peroxidase-conjugated secondary antibody (Vector Laboratories) and SuperSignal West Dura (Pierce) as a chemiluminescent substrate.
To investigate this possibility, we carried out immunoprecipitation assays of MCF-7 nuclear extract with a series of antibodies, and immunoblotted with the same series of antibodies.
Protein samples were resolved on a 10% SDS-PAGE and immunoblotted with primary antibody and revealed with horse radish peroxidase (HRP)-conjugated anti-mouse IgM, or anti-rabbit IgG (Jackson Laboratory, Bar Harbor, Maine, United States).