Words similar to immobilon
Example sentences for: immobilon
How can you use “immobilon” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RNA was transferred to an Immobilon membrane over night in 20 × standard saline citrate (SSC) by capillary action.
Proteins were transferred onto Immobilon P membrane (Millipore), blocked in 5 % nonfat dry milk dissolved in tris-buffered saline / 0.1 % Tween and probed with the various antibodies.
Both types of antibody were affinity purified [ 22 ] using a 6×His fusion to the C-terminal 194 amino acids of Boi1 (the same fusion that was used as immunogen) [ 1 ] . Immobilon P membrane was from Millipore, Inc. (Bedford, MA).
Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.
Proteins were separated by SDS-PAGE through a 12% gel, and then proteins were transferred electrophoretically to Immobilon P membrane.