Example sentences for: immobilon

How can you use “immobilon” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Proteins were separated by SDS-PAGE through a 12% gel, and then proteins were transferred electrophoretically to Immobilon P membrane.

  • Proteins were transferred onto Immobilon P membrane (Millipore), blocked in 5 % nonfat dry milk dissolved in tris-buffered saline / 0.1 % Tween and probed with the various antibodies.

  • Both types of antibody were affinity purified [ 22 ] using a 6×His fusion to the C-terminal 194 amino acids of Boi1 (the same fusion that was used as immunogen) [ 1 ] . Immobilon P membrane was from Millipore, Inc. (Bedford, MA).

  • After SDS-PAGED, proteins were blotted onto nitrocellulose membrane (PVDF Immobilon P Ø 0.45 μm, Millipore).

  • Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.


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