Words similar to depc

Example sentences for: depc

How can you use “depc” in a sentence? Here are some example sentences to help you improve your vocabulary:

Total RNA was extracted from 100 mg of frozen lung using Tri-reagent (Molecular Research Center, Inc., Cincinnati, OH, USA) and the final product was resuspended in 20 μl diethylpyro carbonate depC H 2 O. RNA was quantified and samples with A 260 /A 280 >1.
RNA samples (control and experimental) were each mixed with random octamers in a total volume of 25 μl DEPC (diethylpyrocarbonate) treated water, heated to 65°C for 5 minutes and then transferred to 42°C.
mRNA was added to 4 μg of oligo (dT21) primer (Life Technologies, Rockville, MD), DEPC water was used to bring the volume to 27 μl and the mixture was incubated at 65°C for 10 min and placed on ice.
For the mRNA pans, all solutions and surfaces are pretreated with DEPC or RNaseZap (Ambion, Austin TX), respectively, to eliminate RNase contamination that may compromise the integrity of the RNA.
Samples were diluted in depC H 2 O and 50 ng were used in quantitative one-tube reverse-transcription PCR (Applied Biosystems, Foster City, CA, USA).