Words similar to denatured

Example sentences for: denatured

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Collagen is one of the principal components of the extracellular matrix and RAW cells bind poorly to fibrillar type I collagen unless it is denatured or activated by collagenase [ 38].
Hybridization was performed overnight at 67°C in a sealed bag with 125 μl/sq cm fresh hybridization solution containing formamide and salmon sperm DNA as above with the addition of 40 ng/ml digoxigenin labeled probe denatured by boiling for 5 minutes.
The Cy3 (HL60) and Cy5 (HL60/TLK286) PCR products were purified with Qiagen PCR purification kit, reduced to a final volume of 7.5 μl with a speed vac, mixed with 3.75 μl of 20x SSC, 0.75 μl of 10% SDS, 1.5 μl of 1 μg/μl salmon DNA and 1.5 μl of 50x Denhardt's solution, denatured at 95°C for 5 min, cooled on ice and incubated at 42°C for 15 min.
Hybridization, wash and detection conditions were then applied as above except that chromosomes were denatured by placing slides directly onto a heating block set at 75°C for 7 min.
The blots were pre-hybridized in a solution containing 6-strength SSC (150 mM NaCl, 15 mM Na-citrate, pH 7.0), 0.5% SDS, 50% formamide, and 100 mg/ml denatured sperm DNA at 42°C for 2-4 h, and were hybridized overnight in the same buffer containing approximately 10 6cpm/ml of radiolabeled cDNA probe.