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Example sentences for: bdm
How can you use “bdm” in a sentence? Here are some example sentences to help you improve your vocabulary:
However, BDM has only been shown to inhibit a few myosins in vitro [ 77] and is known to be a nonspecific inhibitor; so these results must be viewed with caution.
Since actin incorporation is not due to elongation of existing filaments and BDM does not affect pure actin dynamics in vitro, we examined the localization of Arp3, a subunit of the actin nucleation Arp2/3 complex.
An important aspect of actin cytoskeletal research has been the use of small molecules that affect actin dynamics and proteins that act on the cytoskeleton [ 1 ] . 2,3-butanedione monoxime (BDM) is a small molecule that inhibits the ATPase activity of muscle myosinII and reduces the force generated by this motor protein [ 2 3 ] . BDM was introduced to the cell biology community as a millimolar inhibitor of multiple non-muscle myosins with greatest potency in inhibiting non-muscle myosinII [ 4 ] . It was subsequently used, in concert with other approaches, to study nm-myosinII function in a variety of biological processes [ 5 6 7 8 ] . BDM was recently shown not to inhibit any non-muscle myosins so far tested, leaving the question of how it affects cells unanswered [ 9 10 ] .
Understanding how these effects are mediated can help us learn from BDM and reinterpret experiments that made use of it, while working to find new and better inhibitors.
BDM has not been exhaustively tested on all myosins, and a myosin target is not unreasonable given findings in Acanthomoeba and S. cerviseae though it does not inhibit Acanthamoeba myosinIc or human myoIe in vitro [ 10 20 21 31 ] . Regardless, we and others have shown that BDM is not the tool that should be used to investigate these behaviours.
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