Words similar to aza-
Example sentences for: aza-
How can you use “aza-” in a sentence? Here are some example sentences to help you improve your vocabulary:
One cell line (T-218A) showed no promoter hypermethylation of MGMT by MSP and no detectable levels of mRNA expression by RT-PCR but expression was reactivated after 5-Aza-2' deoxycytidine treatment.
The MGMT gene exhibited promoter hypermethylation in four of the cell lines, which upon treatment with 5-Aza-2' deoxycytidine showed reactivation of expression in three (2102E-R, 833K-E, and Tera-2).
The SW756 cell line with methylation and one of the two unmethylated cell lines showed reactivated expression upon treatment with 5-Aza-2' deoxycytidine or in combination with n-butyrate, but not with n-butyrate alone.
Cell lines were treated with demethylating agent 5-Aza-2' deoxycytidine (Sigma) for five days at a concentration of 2 to 5 μM, HDAC-inhibiting agent n-butyrate at a final concentration of 5 μM for the last 24 hours or a combination of both.
To further examine the role of promoter methylation in gene inactivation, we treated five NSGCT cell lines (2102E-R, 833K-E, Tera-1, Tera-2, and 218A) with 5-Aza-2' deoxycytidine and analyzed the expression of MGMT , RASSF1A , RARB , and BRCA1 genes.