Words similar to apn
Example sentences for: apn
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Sequence alignments with Plutella xylostella APN receptor indicate that a highly conserved region of APN likely functions as the toxin binding site [ 9 ] . By testing for toxin binding to lysylendopeptidase-digested B. mori APN fragments, the toxin binding site was suggested to be between Ile135 and Pro198.
Bt, Bacillus thuringiensis ; APN, aminopeptidase N; GPI, glycosyl-phosphatidylinositol; BBMV, brush border membrane vesicles; LpNA, leucine-p-nitroanilide; PIPLC, phosphatidylinositol-specific phospholipase C; HBS, Hepes-buffered saline; SPR, surface plasmon resonance
Hybrid toxin 4109 is particular useful in this context, because it is comprised of domains I and II from Cry1Aa and domain III from Cry1Ac (Aa/Aa/Ac) [ 3 ] . Hybrid-toxin 4109 binding to both receptors was not noticeably different from Cry1Aa: for APN binding, k a = 1.9 × 10 4M -1s -1(+/- 1.4 × 10 2), k d = 1.5 × 10 -3s -1(+/- 1.6 × 10 -5), and K D = 78 nM; for cadherin binding, k a = 1.3 × 10 4M -1s -1(+/- 2 × 10 2), k d = 3.34 × 10 -5s -1(+/- 2 × 10 -5), and K D = 2.6 nM.
Similar antibody assays were not performed to substantiate the identity of the purified APN because it clearly displayed strong, characteristic APN enzyme activity.
B. mori APN and B. mori cadherin receptors was evaluated by real-time kinetic analysis on a BIAcore 2000.