Words similar to ampcp
Example sentences for: ampcp
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Figure 3ashows the parallel features of the EPR spectrum of sCD39 bound VO 2+-AMPCP.
However, intact CD39 does not release intermediate ADP during ATP hydrolysis, suggesting that there is only one ADP binding site on each CD39 monomer in the intact protein [ 2 25 ] . The two EPR species observed with VO 2+-AMPCP probably correspond to the two different conformations of bound ADP at the same catalytic site on sCD39.
In order to distinguish species D1 from D2, the sample with VO 2+-AMPCP bound to sCD39 was thawed and incubated at room temperature for 30 minutes, and the VO 2+EPR spectrum was collected again.
In the presence of AMPCP, bound VO 2+produced two EPR features, species D1 and species D2 that are separated by about 30 MHz.
ATP, ADP, ADPNP, AMPCP were purchased from Sigma (St.