Words similar to alphoid
Example sentences for: alphoid
How can you use “alphoid” in a sentence? Here are some example sentences to help you improve your vocabulary:
When centromeric DNA is digested by a restriction endonuclease, the resulting fragments are usually either too small or too large for efficient cloning in bacterial artificial chromosomes [ 22 ] . In recent work we reported isolation of human centromeric DNA using a TAR vector lacking ARS element [ 28 ] . While the isolation was selective, a standard TAR cloning technique does not allow to recover a significant fraction of alphoid DNA arrays that does not contain ARS . In our present study, using the new TAR cloning strategy clones of human alphoid DNA up to ~ 210 kb in length were isolated with high selectivity.
Because the human genome contains approximately 250,000 copies of alphoid DNA monomer, it is possible that alphoid DNA could be cloned into an ARS -containing vector even without counter-selection.
For this purpose, we designed two specific probes - a 56-bp alphoid DNA fragment ( Sat -probe) and a 124-bp Alu fragment ( Alu -probe).
Most of the TAR clones contained exclusively alphoid DNA, which in some cases was organized in higher-order repeat arrays (data not shown).
pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.
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