Example sentences for: alexafluor-

How can you use “alexafluor-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Digital images from one region of the lymphocyte capture membrane were obtained with two different emission filters, one specific for the AlexaFluor-488-conjugated antibody used to stain CD4 + T lymphocytes green (Figure 4A), and the other specific for the AlexaFluor-647-conjugated antibody used to stain CD3 + T lymphocytes red (Figure 4B).

  • For whole blood studies, 33 μl of whole blood collected by venipuncture was incubated at ambient temperature (20–25 °C) with 3 μl of AlexaFluor-488- and AlexaFluor-647-conjugated antibodies to CD4 and CD3 (A21332, clone 289–13801, Molecular Probes), respectively, and allowed to react for 8 min.

  • CD4 cells labeled with AlexaFluor-488-conjugated anti-CD4 antibodies (A21335, clone 289–14120, Molecular Probes, Eugene, Oregon, United States) were introduced to the flow cell in amounts ranging from zero to 200,000 cells, and washed with phosphate buffered saline (PBS).

  • Similarly, for CD8 enumeration, 33 μl of whole blood with 3 μl of AlexaFluor-488- and AlexaFluor-647-conjugated antibodies to CD8 (A21340, clone 289–13804, Molecular Probes) and CD3, respectively, was allowed to react for 8 min at ambient temperature.

  • A similar protocol was applied to a second aliquot of blood stained with AlexaFluor-647-conjugated CD3-specific antibody and AlexaFluor-488-conjugated CD8-specific antibody to visualize and count CD3 + CD8 + T lymphocytes.

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