Example sentences for: aapf-pna

How can you use “aapf-pna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Serial dilutions of the proteases were made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl 2 . Enzyme activity was measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA (Bachem Bioscience, Torrance, CA), 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer.

  • Proteolytic activity was measured by hydrolysis of the substrate succinyl-AAPF-p-nitroanilide (suc-AAPF-pNA).

  • Aliquots of the enzyme solutions were removed over time, diluted and assayed for activity using 1 mM suc-AAPF-pNA substrate in 100 mM Tris pH 8.6 containing 0.0005% Tween-80.


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