Example sentences for: pfa

How can you use “pfa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We have prepared the formaldehyde solution by dissolving PFA in water, because it was pointed out that methanol included in commercial formaldehyde as a preservative, is enough to cause retraction of ruffles and lamellipodia [ 15].

  • Addition of 1% PFA was a necessary compromise to visualize the cytoskeleton and its associated molecules no matter which detergent was being used.

  • Our studies provide first systematic data that cells should be kept in 4% PFA for at least 60 min at room temperature or for 20 min at 37°C to irreversibly crosslink most of the polypeptides (Figure 6).

  • Incubation with sodium borohydride after fixation in PFA had no effect either (data not shown) suggesting that reversible bridges may have formed that could not be reduced.

  • vinculin localized at focal contacts in cells prepared by either PFA/Triton X-100 or by DOTMAC/PFA (Figure 4, B, J, and 4M).


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