Words similar to pevrf-ets-

Example sentences for: pevrf-ets-

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The Ets-1 expression plasmid, pEVRF-ets-1, contains the parental expression plasmid pEVRF0 and the insertion of the Ets-1 cDNA.
We then performed electrophoretic mobility shift assays (EMSA) using radiolabelled Ets oligonucleotide with nuclear extracts prepared from pEVRF-ets-1 transfected M12 cells (figure 3a, lane 3-9).
For the following experiments we used an Ets-1 expression plasmid (pEVRF-ets-1) which encodes Ets-1 protein [ 33, 34].
The pEVRF-ets-1 transfected M12 nuclear extracts form an additional protein:DNA complex (complex 1, figure 3, lane 3) compared with the nuclear extracts from M12 cells transfected with the control plasmid, pEVRF0 (figure 3, lane 2).
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).