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Example sentences for: pet-

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To construct a pBS58 ftsZ mutant plasmid (pBS58 ftsZ mut), the EcoRI and BstEII fragment of ftsZ in pBS58 was replaced with the same part of ftsZ from pET- ftsZ mut.
3, when induced with IPTG, the pET-15b-PfPP5 clone produced a polypeptide of approximate Mr 70 k (lane 2), which is in excellent agreement with the calculated MW of 69,139, considering that pET-15b adds a (His) 6 tag (about 2 kDa) to the N-terminus of the recombinant protein.
The EcoRI and BstEII fragment with a desired mutation from pET- ftsZ mut was put into Litmus38(RI)+H3 precut by the same enzymes.
This sequence was then subcloned into the XhoI and BamHI sites of the pET-15b vector by designing similar primers but containing the respective restriction sites.
After digestion with Nde I and BamH I the coding sequence was subcloned to the vector pET-11a (Stratagene) for gene expression experiments.