Example sentences for: pepstatin

How can you use “pepstatin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Protease inhibitors are 40 μg/ml bestatin, 0.7 μg/ml pepstatin A, 1 mM PMSF, 50 μM leupeptin, 1 mM benzamidine, and 1 mM EGTA.

  • A549 cells or NHBEs were treated with CSC as described above and whole-cell protein extracts were prepared by scraping cells into lysis buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM EDTA, 1 mM sodium orthovanadate, 1 mM sodium fluoride, 1 μg/ml each of leupeptin and pepstatin, and 1 μM PMSF.

  • Cells were harvested by centrifugation and washed once with phosphate-buffered saline (PBS) containing the following protease inhibitors: 2 mg/ml aprotinin, 2 mg/ml leupeptin, 2 mM phenylmethylsulfonyl fluoride (PMSF), and 0.2 mM pepstatin.

  • Control and treated cells were lysed by repeated freeze-thaw cycles with buffer containing 10 mM HEPES (pH 7.5), 1.5 mM Mg(OAc) 2 , 1 mM dithiothreitol (DTT), 0.5% NP40, 5% glycerol, 0.5 mM phenylmethylsulfonyl fluoride, and 10 μg/ml each of the protease inhibitors aprotinin, pepstatin, and leupeptin.

  • Cell were harvested by low-speed centrifugation and resuspended in lysis buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 0.5 M NaCl, 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT).


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