Example sentences for: pem

How can you use “pem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Unspecific epitopes were blocked by incubation in PEM-BAL (PEM plus 1% BSA, 0.1% Na-azide, 0.1 M L-lysine HCl) for 1h.

  • 5 mg/ml, US Biological) in PEM, 1M sorbitol.

  • The taxol-stabilized β-tubulin (1 μM) was also incubated in PEM buffer (100 mM PIPES, 5 mM EGTA, 2 mM MgCl 2 , pH6.

  • Cells were washed in PEM (100 mM Pipes pH6.

  • Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.

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