Example sentences for: pcu

How can you use “pcu” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Immunoblotting of protein lysates prepared from S. pombe cells, whose pcu3 chromosomal locus was modified to encode Pcu3p with thirteen copies of the Myc epitope attached to its C-terminus, revealed two distinct species of Pcu3p, which migrated on SDS gels as two closely spaced species of roughly equal abundance (Fig.

  • Size exclusion chromatography of lysates prepared from cells harboring epitope-tagged Pcu3p or CSN subunits at the endogenous genomic loci, revealed precise co-elution of Pcu3p with CSN subunits 1, 3, 4, and 5 in high molecular weight fractions corresponding to approximately 550 kDa (Fig.

  • We therefore considered the possibility that accumulation of neddylated Pcu3p in csn mutants is a consequence of defective subcellular targeting.

  • In contrast, mutation of a neighboring conserved lysine (residue 760) did not alter the mobility of Pcu3p.

  • We can therefore not exclude that CSN's role in the control of Pcu3p neddylation differs from its role established for Pcu1p [ 10].

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