Words similar to pcu
Example sentences for: pcu
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In contrast, the ubiquitylation assay with Pcu3p-Myc complexes purified from csn5 mutants led to efficient formation of high molecular weight species reactive with ubiquitin antibodies (Fig.
Pcu3p-Myc complexes prepared by immunoprecipitation with Myc antibodies were then incubated with human E1, ATP, ubiquitin, and the recombinant UBCs that interacted with Pip1p.
recently demonstrated that partially purified CSN promotes the cleavage of Pcu1p-Ned8p conjugates in vitro [ 10], but it remained unclear whether the enzymatic activity is contained within one of the CSN subunits or a tightly associated peptidase.
As this shield is experimentally removed through deletion of CSN subunits, Pcu3p may accumulate in the neddylated state.
Several lines of evidence suggest that the SCF pathway is also conserved in the fission yeast Schizosaccharomyces pombe . Pcu1p, a Cdc53p/CUL1-related protein, was shown to associate with two different F-box/WD repeat proteins, Pop1p and Pop2p, when overexpressed [ 18 ] . In addition, genetic studies demonstrated that both of these F-box proteins are simultaneously required for efficient destruction of Rum1p and the replication initiator Cdc18p [ 18 19 20 ] . Rum1p is a Sic1p-analogous CDK inhibitor, which accumulates in G1, but is degraded as cells enter S phase [ 21 22 ] . Failure to degrade Rum1p is the major phenotypic defect of pop1 and pop2 deletion strains, which leads to disturbance of normal cell cycle progression, resulting in polyploidy [ 18 19 20 23 ] . Based on the genetic data and the biochemical observation that Pop1p and Pop2p interact when overexpressed, a putative SCF Pop1p-Pop2pcomplex was proposed, which would contain the heterooligomerizing F-box proteins Pop1p and Pop2p bound to SCF core components [ 18 20 ] .