Words similar to pcrs

Example sentences for: pcrs

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For spectratyping, PCRs were performed as described above with the following VB14- and VB17-specific 5′ primers: VB14m (5′- ACCCAAGATACCTCATCACAG-3′) and VB17 (5′- GACAGGACCCAGGGCAAG-3′), followed by a run-off PCR with downstream VB-specific primers: VB14 (5′- GGGCTTAAGGCAGATCTACT-3′) and VB17m (5′- TTTCAGAAAGGAGATATAGCT-3′), and FAM6-labeled BC63 3′ primer.
The resulting RT reaction products were used to prepare eight 20-μl PCRs per RT reaction.
PCRs with a band of appropriate size were considered positive and scored as 1, negative PCRs as 0 and ambiguous PCRs with bands of unexpected size as 2. The GeneBridge 4 RH map was constructed using a publicly available server at the Whitehead Institute at the Massachusetts Institute of Technology.
The RT reaction was performed using AMV reverse transcriptase, and all PCRs were performed with a 15:1 mixture of Taq:Pfu polymerase.
PCRs were performed in a model 9600 Thermocycler (Perkin-Elmer Cetus) using the following cycling protocol: initial denaturation at 94°C for 10 min; 94.