Example sentences for: pcr-based

How can you use “pcr-based” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • There are several strategies for identifying differentially expressed transcripts, including differential display (DD), representational difference analysis (RDA), serial analysis of gene expression (SAGE), enzymatic degradation subtraction and subtractive hybridization [ 29 30 31 32 33 ] . We chose the PCR-based suppression subtractive hybridization (SSH) procedure for several reasons: it includes a normalization step, it enriches for differentially expressed transcripts, and it yields cDNA fragments that can be used directly for the construction of DNA microarrays.

  • Therefore, we used PCR-based approaches to determine the sequence of the 5' end of the mRNA.

  • Thus, four of the six initial transcripts selected from Affymetrix expression profiling were confirmed as differentially expressed using a PCR-based approach, in a larger sample set from the same clinical trial.

  • PCR-based screening consisted of identifying positive superpools followed by screening of the appropriate plate, row and column pools.

  • Rad26 is a regulatory subunit of the Rad3 checkpoint kinase [ 18 ] . To investigate the cellular distribution of Rad26, we used a one step PCR-based gene-modification method [ 41 ] to fuse GFP (S65T) to the C-terminus of the genomic copy of rad26 +. The Rad26-GFP fusion protein maintained proper checkpoint function following hydroxyurea (HU) and Bleomycin treatments (data not shown).


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