Example sentences for: pcr-amplified

How can you use “pcr-amplified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The sequence of the human midasin gene has been determined from PCR-amplified fragments of cDNA from human testis (Clontech).

  • A sequencing primer was hybridized to the single-stranded copy of the PCR-amplified p53/K- ras 2 fragment and a single base extension with either a Cy-3 or Cy-5 labeled dNTP was performed prior to scanning on a microarray scanner.

  • The cDNA was then PCR-amplified using the primers gcT7a.

  • PCR-amplified products were purified with Gene Clean kits (BIO101, Carlsbad, CA), and eluted in 20 μl water.

  • pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.


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