Example sentences for: pcr-amplified

How can you use “pcr-amplified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Probes were made from PCR-amplified fragments of selected clones using the ReadyPrime random primed DNA labeling kit (Amersham-Pharmacia) with [α- 32P] (ICN Biomedicals, Irvine, CA).

  • Microarrays with approximately 43,000 sequence-validated PCR-amplified human cDNAs representing 32,968 UniGene clusters were manufactured as described [ 32 ] . Hybridizations were performed using equal amounts of Cy3- and Cy5-labeled cDNAs according to previously published protocols [ 32 ] . Hybridized microarrays were scanned using a GenePix4000 array scanner and analyzed with GenePix Pro 3.0 software (Axon Instruments, Union City, CA).

  • These include: the glass-slide based oligonucleotide array system [ 2, 3, 4] developed by Affymetrix; the glass-slide-based cDNA clone Gene Expression Microarray (GEM) line from Incyte Genomics (IGI); the filter and glass-slide-based 'Atlas' arrays developed by Clontech Laboratories, composed of selected regions of PCR-amplified gene sequences; filter-based GeneFilters from Research Genetics (RGI) which, like IGI's GEMs, utilize partial or full-length cDNA clones.

  • pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.

  • The PGMinvrpt construct was made as follows: because the final insert would be a large inverted repeat, Pgm Swas PCR-amplified in two pieces, in opposite orientations.


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