Example sentences for: pci-neo

How can you use “pci-neo” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The vector pGAL4-EGFP was constructed from pCI-neo (Promega) by replacing the CMV promoter and enhancer with a fragment from pG5-luc (Promega) containing the 5 × GAL4 recognition sequences and the minimal promoter from the adenoviral late promoter and the EGFP gene from pEGFP (Clontech).

  • The CMV promoter and enhancer were removed from the mammalian expression vector pCI-neo (Promega).

  • Stably transfected HEK cells were developed as described previously [ 33 ] . HEK 293 cells were transfected with the cDNA for mouse δ opioid receptor in the pCI-neo ®vector.

  • The 1.2 kb coding sequence of the receptor was subcloned into pCI-neo ®mammalian expression vector (Promega, Madison, WI) using the restriction sites EcoRI and Sal I. HEK 293 cell were transfected with the vector using the lipofectin ®reagent (Life Technologies, Rockville, MD).

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